abstract：:Type 2 diabetes, characterized by peripheral target tissue resistance to insulin, is epidemic in industrialized countries and is strongly associated with obesity. The protein hormone, resistin, secreted specifically by the adipose tissues, is found to antagonize insulin action upon glucose uptake and may serve as an i...

journal_title：Journal of biotechnology

authors： Juan CC,Kan LS,Huang CC,Chen SS,Ho LT,Au LC

更新日期：2003-06-26 00:00:00

abstract：:Human interleukin-10 (hIL-10) has wide spectrum of anti-inflammatory activities and has shown a potential to be used for treatment of inflammatory or immune illness. In this study, transgenic mice that over-express human interleukin-10 (IL-10) in their milk were generated using a bovine beta-casein/human IL-10 hybrid ...

journal_title：Journal of biotechnology

authors： Sohn BH,Chang HG,Kang HS,Yoon H,Bae YS,Lee KK,Kim SJ

更新日期：2003-06-12 00:00:00

abstract：:One of the major constraints to performing large-scale transfections of cultured mammalian cells for the transient expression of recombinant proteins is the production of large quantities of purified plasmid DNA. In this report partially purified plasmid DNA was prepared by a method that combines alkaline lysis of E. ...

journal_title：Journal of biotechnology

authors： Wright JL,Jordan M,Wurm FM

更新日期：2003-05-08 00:00:00

abstract：:The rare earth elements Nd, La, Ce at proper concentrations had positive effects on the cell growth of Cistanche deserticola and production of phenylethanoid glycosides (PeG). A mixture of rare earth elements (MRE, La(2)O(3):CeO(2):Pr(6)O(11):Sm(2)O(3)=255:175:3:1, mol/mol) showed the most remarkable effects. After 30...

journal_title：Journal of biotechnology

authors： Ouyang J,Wang X,Zhao B,Yuan X,Wang Y

更新日期：2003-04-24 00:00:00

abstract：:In this paper, single-stranded (ss)DNA aptamers with capability to distinguish differentiated PC12 cells from normal PC12 cells were selected by subtractive systematic evolution of ligands by exponential enrichment (SELEX) method. Before each round of selection, randomized ssDNAs were incubated with regular PC12 cells...

journal_title：Journal of biotechnology

authors： Wang C,Zhang M,Yang G,Zhang D,Ding H,Wang H,Fan M,Shen B,Shao N

更新日期：2003-04-10 00:00:00

abstract：:GeneTag is a novel expression profiling method that allows the visualization, quantification and identification of expressed genes-whether known or novel-in any species, tissue or cell type, independent of knowledge of the underlying sequence. Here we describe the application of this method to determine variation of g...

journal_title：Journal of biotechnology

authors： Wong LY,Hafeman A,Boyd VL,Bodeau J,Lazaruk KD,Liew SN,Casey P,Belonogoff V,Bit S,Sumner C,Bredo A,Ho N,Chu E,Olson S,Rabkin S,Maltchenko S,Spier G,Gilbert D,Baumhueter S

更新日期：2003-03-20 00:00:00

abstract：:A new algorithm was developed for the estimation of the metabolic flux distribution based on GC-MS data of proteinogenic amino acids. By using a sensitive GC-MS protocol as well as by combining the global search algorithm such as the genetic algorithm with the local search algorithm such as the Levenberg-Marquardt alg...

journal_title：Journal of biotechnology

authors： Zhao J,Shimizu K

更新日期：2003-03-06 00:00:00

abstract：:Six bacterial strains with the capability of degrading textile dyes were isolated from sludge samples and mud lakes. Aeromonas hydrophila was selected and identified because it exhibited the greatest color removal from various dyes. Although A. hydrophila displayed good growth in aerobic or agitation culture (AGI cult...

journal_title：Journal of biotechnology

authors： Chen KC,Wu JY,Liou DJ,Hwang SC

更新日期：2003-02-27 00:00:00

abstract：:A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) is a novel family of extracellular proteases supposedly involved in inflammation, angiogenesis, development and coagulation. To overexpress the active ADAMTS proteins, we designed a chimeric molecule composed of a catalytic domain of ADAMTS-1...

journal_title：Journal of biotechnology

authors： Sugiura T

更新日期：2003-02-13 00:00:00

abstract：:The establishment and optimization of in vitro primmorph formation from a Chinese sponge, Stylotella agminata (Ridley), collected from the South China Sea, were investigated. Our aims were to identify the key factors affecting primmorph formation in this species and to optimize the technique for developing an in vitro...

journal_title：Journal of biotechnology

authors： Zhang W,Zhang X,Cao X,Xu J,Zhao Q,Yu X,Jin M,Deng M

更新日期：2003-01-23 00:00:00

abstract：:In this paper, the feasibility of precipitation driven synthesis of acidic and zwitterionic beta-lactam antibiotics is studied. As an example of the first type, penicillin G was produced in good yield (160 mmol kg(-1)) directly from the free acid and amine aqueous substrate suspension, where the synthesis product prec...

journal_title：Journal of biotechnology

authors： Ulijn RV,De Martin L,Halling PJ,Moore BD,Janssen AE

更新日期：2002-11-13 00:00:00

abstract：:A cellulase from Trichoderma reesei was immobilized on Eudragit L-100, a reversibly soluble polymer depending on the pH of the medium. The solubility of the modified cellulase was studied at different pH values. By changing the pH, the adsorption equilibrium of the derivatized proteins is switched towards the liquid p...

journal_title：Journal of biotechnology

authors： Dourado F,Bastos M,Mota M,Gama FM

更新日期：2002-10-23 00:00:00

abstract：:Protein secretion from Bacillus species is a major industrial production tool with a market of over $1 billion per year. However, standard export technologies, based on the well-characterised general secretory (Sec) pathway, are frequently inapplicable for the production of proteins. The recently discovered twin-argin...

journal_title：Journal of biotechnology

authors： van Dijl JM,Braun PG,Robinson C,Quax WJ,Antelmann H,Hecker M,Müller J,Tjalsma H,Bron S,Jongbloed JD

更新日期：2002-09-25 00:00:00

abstract：:Environmental biotechnology, until now, has primarily focused on the development of technologies to treat aqueous, solid and gaseous wastes. At present, the basic knowledge on how biotechnology can handle these wastes has been acquired and the focus is now on the implementation of these processes as 'best available te...

journal_title：Journal of biotechnology

authors： Grommen R,Verstraete W

更新日期：2002-09-11 00:00:00

abstract：:To monitor the colonization of wheat roots by Azospirillum brasilense, we constructed several plasmids based on the pBBR1 replicon expressing the gfp and gusA genes constitutively. Both genes were placed under control of the gentamycin resistance gene promoter resulting in high levels of expression in Escherichia coli...

journal_title：Journal of biotechnology

authors： Ramos HJ,Roncato-Maccari LD,Souza EM,Soares-Ramos JR,Hungria M,Pedrosa FO

更新日期：2002-08-28 00:00:00

abstract：:Methods of adapting micro-organisms to an inhibiting factor in an active industrial bioprocess were examined with an acetic acid fermentation as model. With the aim of automatic control, a fuzzy-logic system was developed on the basis of the collected knowledge of skilled vinegar brewers. In a first step, this fuzzy s...

journal_title：Journal of biotechnology

authors： Arnold S,Becker T,Delgado A,Emde F,Enenkel A

更新日期：2002-08-07 00:00:00

abstract：:Genetically engineered human osteosarcoma cells containing developmental endothelial locus-1 (del-1) gene were studied for production of Del-1, a protein that has the properties of an extracellular matrix protein and can regulate vascular morphogenesis and remodeling. Del-1 has been studied as a potential anti-angioge...

journal_title：Journal of biotechnology

authors： Chen C,Huang YL,Yang ST

更新日期：2002-07-17 00:00:00

abstract：:A novel method for immobilizing large DNA fragments on a solid surface was developed. A mixed self-assembled monolayer of thiolated single-stranded DNA with inert alkanethiol was generated on a gold (Au) surface through the Au-S reaction. Surface-tethered DNA generated by this method was compatible with various geneti...

journal_title：Journal of biotechnology

authors： Kim JH,Hong JA,Yoon M,Yoon MY,Jeong HS,Hwang HJ

更新日期：2002-07-03 00:00:00

abstract：:In this article, we review how molecular modeling techniques can be used to shed light on how water and organic solvents influence the reactivity of enzymes. The application of thermodynamics-based models allowed the first qualitative predictions on the selectivity of many reaction types. However, it was with the appl...

journal_title：Journal of biotechnology

authors： Colombo G,Carrea G

更新日期：2002-06-13 00:00:00

abstract：:We have carried out the adaptation of BHK-21 cells to two serum free (Ex Cell 520 and HyQ PF CHO) and three animal protein free media: Ex Cell 302, HyQ PF CHO MPS and Rencyte BHK. After a direct switch or a gradual adaptation, we have achieved BHK-21 cells growth in the following media: HyQ PF CHO, HyQ PF CHO MPS, Ren...

journal_title：Journal of biotechnology

authors： Kallel H,Jouini A,Majoul S,Rourou S

更新日期：2002-05-23 00:00:00

abstract：:Glycodendrimers are relatively novel synthetic biomacromolecules that are made of biologically relevant carbohydrate ligands constructed at the periphery of a wide range of highly functionalized and repetitive scaffolds having varied molecular weights and structures. They were aimed to fill the gap between glycopolyme...

journal_title：Journal of biotechnology

authors： Roy R,Baek MG

更新日期：2002-05-01 00:00:00

abstract：:An assay was developed to measure the hydrophobic interactions of commonly used mammalian cell lines grown in culture. The assay depends on the loss of cells from an aqueous suspension following vortexing with a hydrophobic oil phase. This allowed the determination of a hydrophobicity index, which was significantly hi...

journal_title：Journal of biotechnology

authors： Ghebeh H,Gillis J,Butler M

更新日期：2002-04-25 00:00:00

abstract：:Magnetic particles produced by magnetic bacteria have been used to carry out magnetic separation of DNA. Separation was achieved using magnetite coated with 3-aminopropyltriethoxysilane, N-(trimethoxy-silylpropyl) isothiouronium chloride or 3-[2-(2-aminoethyl)-ethylamino]-propyltrimethoxysilane (AEEA). The DNA binding...

journal_title：Journal of biotechnology

authors： Yoza B,Matsumoto M,Matsunaga T

更新日期：2002-04-11 00:00:00

abstract：:To improve tumor-to-tissue ratios of anticancer agents in radioimmunotherapy, a three-step targeting approach was used to deliver biotinylated liposomes to human ovarian cancer cells (NIH:OVCAR-3, SK-OV-3) in vitro. Targeting was based upon the use of two antibodies specific for the CA-125 antigen that is highly expre...

journal_title：Journal of biotechnology

authors： Xiao Z,McQuarrie SA,Suresh MR,Mercer JR,Gupta S,Miller GG

更新日期：2002-03-28 00:00:00

abstract：:The first problem in gene expression profiling to be solved is choosing the appropriate gene array, detection procedure, image analysis and data generation depending on the organism of interest, equipment and budget. The next one is how to deduce biologically meaningful data. We assessed gene expression data from chem...

journal_title：Journal of biotechnology

authors： Fahnert B,Hahn D,Guthke R

更新日期：2002-03-14 00:00:00

abstract：:Recently, a recombinant yeast pyruvate carboxylase expressed in the cytoplasm of BHK-21 cells was shown to reconstitute the missing link between glycolysis and TCA, thus increasing the flux of glucose into the TCA and resulting in a higher intracellular ATP content. Now, these metabolically engineered cells have been ...

journal_title：Journal of biotechnology

authors： Irani N,Beccaria AJ,Wagner R

更新日期：2002-02-28 00:00:00

abstract：:A gene encoding for hirudin, a potent thrombin inhibitor, was expressed in Escherichia coli, which is the most widely used host. When the recombinant hirudin analog, CX-397, was overproduced by E. coli (600 mg l(-1)) in the absence of nutrient amino acids in the culture medium, the presence of two derivatives in the f...

journal_title：Journal of biotechnology

authors： Muramatsu R,Negishi T,Mimoto T,Miura A,Misawa S,Hayashi H

更新日期：2002-02-14 00:00:00

abstract：:Development of reagentless biosensors implies the tight and functional immobilisation of biological recognition elements on transducer surfaces. Specifically, in the case of amperometric enzyme electrodes, electron-transfer pathways between the immobilised redox protein and the electrode surface have to be established...

journal_title：Journal of biotechnology

authors： Schuhmann W

更新日期：2002-02-01 00:00:00

abstract：:Aqueous two-phase systems allow for the unequal distribution of proteins and other molecules in water-rich solutions containing phase separating polymers or surfactants. One approach to improve the partitioning properties of recombinant proteins is to produce the proteins as fused to certain peptide tags. However, the...

journal_title：Journal of biotechnology

authors： Bandmann N,Van Alstine J,Veide A,Nygren PA

更新日期：2002-01-31 00:00:00

abstract：:The unsaturations and groups bound to the ring and to the lateral chain of lactones give a large diversity in this class of molecules. In this work we produced enzymatically a macrolactone in organic media. The substrate used was a hydroxy-fatty acid: (+)-coriolic acid and the enzymes tested were free or immobilized m...

journal_title：Journal of biotechnology

authors： Gargouri M,Drouet P,Legoy MD

更新日期：2002-01-18 00:00:00

abstract：:Fluorescence resonance energy transfer studies allow to determine global shape properties of nucleic acids and nucleoprotein complexes. In many DNA-protein complexes, the DNA is more or less bent and the degree of bending can be obtained by FRET. For example, the DNA in complex with the integration host factor (IHF) i...

journal_title：Journal of biotechnology

authors： Lorenz M,Hillisch A,Diekmann S

更新日期：2002-01-01 00:00:00

abstract：:A newly developed flat panel airlift photobioreactor with a defined circulation path was tested for microalgal culture. The bioreactor exposed the cells to intermittent light to improve the efficiency of light utilization through the flashing-light effect. During batch cultures in the new photobioreactor, the biomass ...

journal_title：Journal of biotechnology

authors： Degen J,Uebele A,Retze A,Schmid-Staiger U,Trösch W

更新日期：2001-12-28 00:00:00

abstract：:Pure wine yeast cultures are increasingly used in winemaking to perform controlled fermentations and produce wine of reproducible quality. For the genetic manipulation of natural wine yeast strains dominant selective markers are obviously useful. Here we demonstrate the successful use of the mutated PDR3 gene as a dom...

journal_title：Journal of biotechnology

authors： Kozovska Z,Maraz A,Magyar I,Subik J

更新日期：2001-12-14 00:00:00

abstract：:The miniaturization of analytical devices by micromachining technology is destined to have a major impact on medical and bioanalytical fields. To meet the current demands for rapid DNA amplification, various instruments and innovative technologies have been introduced by several groups in recent years. The development...

journal_title：Journal of biotechnology

authors： Schneegass I,Köhler JM

更新日期：2001-12-01 00:00:00

abstract：:A rapid quantitative analysis method for murine endothelin-1 (ET-1) and vasoactive intestinal contractor (VIC) gene expression levels was established using a real-time polymerase chain reaction (PCR). We designed primer pairs and TaqMan probes specific for murine prepro-ET-1 (PPET-1) and prepro-VIC (PPVIC) genes, base...

journal_title：Journal of biotechnology

authors： Uchide T,Adur J,Saida K

更新日期：2001-11-30 00:00:00

abstract：:Inoculation of naked DNA has been applied for the development of prophylactic and therapeutic vaccines against different viral infections. To study the humoral immune response induced by DNA vaccination we cloned the entire nucleocapsid protein-encoding sequence of the Puumala hantavirus strain Vranica/Hällnäs into th...

journal_title：Journal of biotechnology

authors： Koletzki D,Schirmbeck R,Lundkvist A,Meisel H,Krüger DH,Ulrich R

更新日期：2001-11-17 00:00:00

abstract：:The investigation of bio-molecules has entered a new age since the development of methodologies capable of studies at the level of single molecules. In biology, most molecules show a complex dynamical behavior, with individual motions and transitions between different states, occurring as highly correlated in space an...

journal_title：Journal of biotechnology

authors： Hinterdorfer P,Schütz G,Kienberger F,Schindler H

更新日期：2001-11-01 00:00:00

abstract：:Herbaspirillum seropedicae is a nitrogen-fixing bacterium found in association with economically important gramineae. Regulation of nitrogen fixation involves the transcriptional activator NifA protein. The regulation of NifA protein and its truncated mutant proteins is described and compared with that of other nitrog...

journal_title：Journal of biotechnology

authors： Pedrosa FO,Benelli EM,Yates MG,Wassem R,Monteiro RA,Klassen G,Steffens MB,Souza EM,Chubatsu LS,Rigo LU

更新日期：2001-10-04 00:00:00

abstract：:A pair of genes designated parA and parB are encoded by many low copy number plasmids and bacterial chromosomes. They work with one or more cis-acting sites termed centromere-like sequences to ensure better than random predivisional partitioning of the DNA molecule that encodes them. The centromere-like sequences nucl...

journal_title：Journal of biotechnology

authors： Bignell C,Thomas CM

更新日期：2001-09-13 00:00:00

abstract：:The combination of anaerobic and aerobic periods in the operation cycle of a Sequencing Batch Reactor (SBR) was chosen to study biological color removal from simulated textile effluents containing reactive, sulfonated, monoazo and diazo dyes, respectively, Remazol Brilliant Violet 5R and Remazol Black B. 90% color rem...

journal_title：Journal of biotechnology

authors： Lourenço ND,Novais JM,Pinheiro HM

更新日期：2001-08-23 00:00:00